| GIORNATA | PARTITA | RISULTATO | DATA | LUOGO | | |
|---|
| 1 | Lazio – Atalanta | 1 – 4 | 30/09/2020 – 20:45 | OLIMPICO – Roma | | |
| 2 | Cagliari – Lazio | 0 – 2 | 26/09/2020 – 18:00 | SARDEGNA ARENA – Cagliari | | |
| 3 | Lazio – Inter | 1 – 1 | 04/10/2020 – 15:00 | OLIMPICO – Roma | | |
| 4 | Sampdoria – Lazio | 3 – 0 | 17/10/2020 – 18:00 | LUIGI FERRARIS – Genova | | |
| 5 | Lazio – Bologna | 3-1 | 24/10/2020 – 20:45 | OLIMPICO – Roma | | |
| 6 | Torino – Lazio | 3-4 | 01/11/2020 – 15:00 | OLIMPICO GRANDE TORINO – Torino | | |
| 7 | Lazio – Juventus | 1-1 | 08/11/2020 – 12:30 | OLIMPICO – Roma | | |
| 8 | Crotone – Lazio | 0-2 | 21/11/2020 – 15:00 | EZIO SCIDA – Crotone | | |
| 9 | Lazio – Udinese | 1-3 | 29/11/2020 – 12:30 | OLIMPICO – Roma | | |
| 10 | Spezia – Lazio | 1-2 | 05/12/2020 – 15:00 | OROGEL STADIUM – Cesena | | |
| 11 | Lazio – Hellas Verona | 1-2 | 12/12/2020 – 20:45 | OLIMPICO – Roma | | |
| 12 | Benevento – Lazio | 1-1 | 15/12/2020 – 20:45 | CIRO VIGORITO – Benevento | | |
| 13 | Lazio – Napoli | 2-0 | 20/12/2020 – 20:45 | OLIMPICO – Roma | | |
| 14 | Milan – Lazio | 3-2 | 23/12/2020 – 20:45 | GIUSEPPE MEAZZA – Milano | | |
| 15 | Genoa – Lazio | 1-1 | 03/01/2021 – 15:00 | LUIGI FERRARIS – Genova | | |
| 16 | Lazio – Fiorentina | 2-1 | 06/01/2021 – 15:00 | OLIMPICO – Roma | | |
| 17 | Parma – Lazio | 0-2 | 10/01/2021 – | ENNIO TARDINI – Parma | | |
| 18 | Lazio – Roma | 3-0 | 17/01/2021 – | OLIMPICO – Roma | | |
| 19 | Lazio – Sassuolo | – | 24/01/2021 – | OLIMPICO – Roma | | |
| 20 | Atalanta – Lazio | – | 31/01/2021 – | GEWISS STADIUM – Bergamo | | |
| 21 | Lazio – Cagliari | – | 07/02/2021 – | OLIMPICO – Roma | | |
| 22 | Inter – Lazio | – | 14/02/2021 – | GIUSEPPE MEAZZA – Milano | | |
| 23 | Lazio – Sampdoria | – | 21/02/2021 – | OLIMPICO – Roma | | |
| 24 | Bologna – Lazio | – | 28/02/2021 – | RENATO DALL’ARA – Bologna | | |
| 25 | Lazio – Torino | – | 03/03/2021 – | OLIMPICO – Roma | | |
| 26 | Juventus – Lazio | – | 06/03/2021 – 20:45 | ALLIANZ STADIUM – Torino | | |
| 27 | Lazio – Crotone | – | 14/03/2021 – | OLIMPICO – Roma | | |
| 28 | Udinese – Lazio | – | 21/03/2021 – | DACIA ARENA – STADIO FRIULI – Udine | | |
| 29 | Lazio – Spezia | – | 03/04/2021 – | OLIMPICO – Roma | | |
| 30 | Hellas Verona – Lazio | – | 11/04/2021 – | MARCANTONIO BENTEGODI – Verona | | |
| 31 | Lazio – Benevento | – | 18/04/2021 – | OLIMPICO – Roma | | |
| 32 | Napoli – Lazio | – | 21/04/2021 – | SAN PAOLO – Napoli | | |
| 33 | Lazio – Milan | – | 25/04/2021 – | OLIMPICO – Roma | | |
| 34 | Lazio – Genoa | – | 02/05/2021 – | OLIMPICO – Roma | | |
| 35 | Fiorentina – Lazio | – | 09/05/2021 – | ARTEMIO FRANCHI – Firenze | | |
| 36 | Lazio – Parma | – | 12/05/2021 – | OLIMPICO – Roma | | |
| 37 | Roma – Lazio | – | 16/05/2021 – | OLIMPICO – Roma | | |
| 38 | Sassuolo – Lazio | – | 23/05/2021 – | MAPEI STADIUM – Reggio Emilia | | |
